More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/RIT2 Mouse Monoclonal Antibody [Clone ID: OTI3F4]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIT2 (RC205367, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIT2.(1:500) This antibody recognizes a nonspecific band around 20KD
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIT2 (RC205367, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIT2.(1:500) This antibody recognizes a nonspecific band around 20KD
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIT2 (RC205367, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIT2.(1:500) This antibody recognizes a nonspecific band around 20KD

RIT2 Mouse Monoclonal Antibody [Clone ID: OTI3F4]

CF501757
OriGene
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetRIT2
Price on request
100 ug
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    RIT2 Mouse Monoclonal Antibody [Clone ID: OTI3F4]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3F4
  • Gene ID6014
  • Target name
    RIT2
  • Target description
    Ras like without CAAX 2
  • Target synonyms
    RIBA, RIN, ROC2, GTP-binding protein Rit2, GTP-binding protein Roc2, Ric-like, expressed in neurons, ras-like protein expressed in neurons, ras-like without CAAX protein 2
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ99578
  • Protein Name
    GTP-binding protein Rit2
  • Scientific Description
    Carrier-free (BSA/glycerol-free) RIT2 mouse monoclonal antibody, clone OTI3F4 (formerly 3F4)
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Figure 1. Western blot analysis of RIT2 using anti-RIT2 antibody (A07833-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human SK-N-SH whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIT2 antigen affinity purified polyclonal antibody (Catalog # A07833-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIT2 at approximately 17,21 kDa. The expected band size for RIT2 is at 25 kDa.
Product group Antibodies
Boster Bio
Anti-RIT2 Antibody Picoband(r)A07833-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetRIT2
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
RIT2 / RIN AntibodyLS-C748106
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetRIT2
  • SizePrice
Immunohistochemical staining of human cerebellum shows moderate positivity in neuronal processes in molecular layer.
Product group Antibodies
Atlas Antibodies
Anti-RIT2 AntibodyHPA072174
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetRIT2
  • SizePrice
Immunofluorescence staining of A549 cells with CSB-PA860769LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Product group Antibodies
Cusabio
RIT2 AntibodyCSB-PA860769LA01HU
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetRIT2
  • SizePrice
WB analysis of various cell lines using GTX83710 RIT2 antibody [3F4]. Loading : 35 ug per lane
Product group Antibodies
GeneTex
RIT2 antibody [3F4]GTX83710
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityCanine, Human, Monkey, Rat
TargetRIT2
  • SizePrice