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Immunohistochemistry of paraffin-embedded human eye tissue using CSB-PA019743LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human eye tissue using CSB-PA019743LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human eye tissue using CSB-PA019743LA01HU at dilution of 1:100

RLBP1 Antibody

CSB-PA019743LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRLBP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    RLBP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6017
  • Target name
    RLBP1
  • Target description
    retinaldehyde binding protein 1
  • Target synonyms
    cellular retinaldehyde-binding protein; cellular retinaldehyde-binding protein-1; CRALBP; retinaldehyde-binding protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12271
  • Protein Name
    Retinaldehyde-binding protein 1
  • Scientific Description
    Soluble retinoid carrier essential the proper function of both rod and cone photoreceptors. Participates in the regeneration of active 11-cis-retinol and 11-cis-retinaldehyde, from the inactive 11-trans products of the rhodopsin photocycle and in the de novo synthesis of these retinoids from 11-trans metabolic precursors. The cycling of retinoids between photoreceptor and adjacent pigment epithelium cells is known as the visual cycle.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RLBP1 using anti-RLBP1 antibody (A05421-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RLBP1 antigen affinity purified polyclonal antibody (Catalog # A05421-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RLBP1 at approximately 36 kDa. The expected band size for RLBP1 is at 36 kDa.
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Immunohistochemical staining of human eye, retina, liver, lymphoid tissues and squamous epithelia using Anti-RLBP1 antibody HPA044083 (A) shows similar protein distribution across tissues to independent antibody HPA042051 (B).
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