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Western Blot Positive WB detected in: Hela whole cell lysate, PC-3 whole cell lysate All lanes: RNASEH1 antibody at 3.59microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 33 kDa Observed band size: 33 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, PC-3 whole cell lysate All lanes: RNASEH1 antibody at 3.59microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 33 kDa Observed band size: 33 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, PC-3 whole cell lysate All lanes: RNASEH1 antibody at 3.59microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 33 kDa Observed band size: 33 kDa

RNASEH1 Antibody

CSB-PA019801LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRNASEH1
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Overview

  • Supplier
    Cusabio
  • Product Name
    RNASEH1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID246243
  • Target name
    RNASEH1
  • Target description
    ribonuclease H1
  • Target synonyms
    H1RNA; PEOB2; ribonuclease H type II; ribonuclease H1; RNH1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60930
  • Protein Name
    Ribonuclease H1
  • Scientific Description
    Endonuclease that specifically degrades the RNA of RNA-DNA hybrids (PubMed:10497183). Plays a role in RNA polymerase II (RNAp II) transcription termination by degrading R-loop RNA-DNA hybrid formation at G-rich pause sites located downstream of the poly(A) site and behind the elongating RNAp II (PubMed:21700224).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RNASEH1 using anti-RNASEH1 antibody (A06342-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNASEH1 antigen affinity purified polyclonal antibody (Catalog # A06342-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNASEH1 at approximately 38 kDa. The expected band size for RNASEH1 is at 32 kDa.
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