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ROCK1 antibody [N1N2], N-term

GTX113266
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetROCK1
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Overview

  • Supplier
    GeneTex
  • Product Name
    ROCK1 antibody [N1N2], N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.35 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6093
  • Target name
    ROCK1
  • Target description
    Rho associated coiled-coil containing protein kinase 1
  • Target synonyms
    p160 ROCK-1; P160ROCK; renal carcinoma antigen NY-REN-35; rho-associated protein kinase 1; ROCK-I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13464
  • Protein Name
    Rho-associated protein kinase 1
  • Scientific Description
    This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Induction of osteogenic differentiation of bone marrow stromal cells on 3D polyester-based scaffolds solely by subphysiological fluidic stimulation in a laminar flow bioreactor. Yamada S et al., 2021 Jan-Dec, J Tissue Eng
    Read more
  • Effect of Tissue Transglutaminase on Steroid Resistance in T-Cell Acute Lymphoblastic Leukemia. Jung HJ et al., 2019 Nov, Anticancer Res
    Read more
  • Up regulation of Rho-associated coiled-coil containing kinase1 (ROCK1) is associated with genetic instability and poor prognosis in prostate cancer. Steurer S et al., 2019 Sep 25, Aging (Albany NY)
    Read more
  • Cholix toxin, an eukaryotic elongation factor 2 ADP-ribosyltransferase, interacts with Prohibitins and induces apoptosis with mitochondrial dysfunction in human hepatocytes. Yahiro K et al., 2019 Aug, Cell Microbiol
    Read more
  • Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation. Tsuchiya M et al., 2018 May 24, Nat Commun
    Read more
  • Estrogen-related receptor alpha decreases RHOA stability to induce orientated cell migration. Sailland J et al., 2014 Oct 21, Proc Natl Acad Sci U S A
    Read more
  • Androgen-regulated microRNA-135a decreases prostate cancer cell migration and invasion through downregulating ROCK1 and ROCK2. Kroiss A et al., 2015 May 28, Oncogene
    Read more

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Figure 1. Western blot analysis of ROCK1 using anti-ROCK1 antibody (A00722-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK1 antigen affinity purified polyclonal antibody (Catalog # A00722-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROCK1 at approximately 160 kDa. The expected band size for ROCK1 is at 158 kDa.
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