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ROR gamma antibody

GTX108728
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetRORC
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Overview

  • Supplier
    GeneTex
  • Product Name
    ROR gamma antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6097
  • Target name
    RORC
  • Target description
    RAR related orphan receptor C
  • Target synonyms
    IMD42; NR1F3; nuclear receptor ROR-gamma; nuclear receptor RZR-gamma; nuclear receptor subfamily 1 group F member 3; RAR-related orphan nuclear receptor variant 2; retinoic acid-binding receptor gamma; retinoid-related orphan receptor gamma; RORG; RZRG; RZR-GAMMA; TOR
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51449
  • Protein Name
    Nuclear receptor ROR-gamma
  • Scientific Description
    The protein encoded by this gene is a DNA-binding transcription factor and is a member of the NR1 subfamily of nuclear hormone receptors. The specific functions of this protein are not known; however, studies of a similar gene in mice have shown that this gene may be essential for lymphoid organogenesis and may play an important regulatory role in thymopoiesis. In addition, studies in mice suggest that the protein encoded by this gene may inhibit the expression of Fas ligand and IL2. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Mucosal Immunity Related to FOXP3+ Regulatory T Cells, Th17 Cells and Cytokines in Pediatric Inflammatory Bowel Disease. Cho J et al., 2018 Dec 24, J Korean Med Sci
    Read more

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Figure 1. Western blot analysis of ROR gamma/RORC using anti-ROR gamma/RORC antibody (A00444-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROR gamma/RORC antigen affinity purified polyclonal antibody (Catalog # A00444-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROR gamma/RORC at approximately 58 kDa. The expected band size for ROR gamma/RORC is at 58 kDa.
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