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RPB16 / POLR2D Antibody

LS-C817016
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetPOLR2D
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RPB16 / POLR2D Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    IP, WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5433
  • Target name
    POLR2D
  • Target description
    RNA polymerase II subunit D
  • Target synonyms
    HSRBP4, HSRPB4, RBP4, RPB16, RPB4, DNA-directed RNA polymerase II subunit RPB4, DNA-directed RNA polymerase II 16 kDa polypeptide, DNA-directed RNA polymerase II subunit D, RNA polymerase II 16 kDa subunit, RNA polymerase II subunit B4, RNA polymerase II subunit hsRBP4, RNA polymerase II subunit hsRPB4, polymerase (RNA) II (DNA directed) polypeptide D, polymerase (RNA) II subunit D
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POLR2D using anti-POLR2D antibody (A12839-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR2D antigen affinity purified polyclonal antibody (Catalog # A12839-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR2D at approximately 18 kDa. The expected band size for POLR2D is at 16-20 kDa.
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