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RPL17 Antibody Pair

CSB-EAP02504
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityCanine, Guinea Pig, Human, Porcine, Rabbit
TargetRPL17
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Overview

  • Supplier
    Cusabio
  • Product Name
    RPL17 Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 0.5ug/ml and the detection antibody at a concentration of 0.125ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID6139
  • Target name
    RPL17
  • Target description
    ribosomal protein L17
  • Target synonyms
    DBA22, L17, PD-1, RPL23, uL22, large ribosomal subunit protein uL22, 60S ribosomal protein L17, 60S ribosomal protein L23, gene encoding putative NFkB activating protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18621
  • Protein Name
    Large ribosomal subunit protein uL22
  • Reactivity
    Canine, Guinea Pig, Human, Porcine, Rabbit
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of RPL17 using anti-RPL17 antibody (A06980-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat pancreas tissue lysates, Lane 6: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL17 antigen affinity purified polyclonal antibody (Catalog # A06980-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL17 at approximately 21 kDa. The expected band size for RPL17 is at 21 kDa.
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