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Western blot analysis of extracts of various cell lines using RPL27 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using RPL27 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using RPL27 Polyclonal Antibody at dilution of 1:3000.

RPL27 Polyclonal Antibody

E-AB-64427
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    RPL27 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L27E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RPL27 using anti-RPL27 antibody (A09736-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human RT4 whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 8: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL27 antigen affinity purified polyclonal antibody (Catalog # A09736-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL27 at approximately 16 kDa. The expected band size for RPL27 is at 16 kDa.
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Western blot All lanes: RPL27 antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa
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WB analysis of various sample lysates using GTX66153 RPL27 antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:3000 Loading : 25μg per lane
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