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Western blot All lanes: RPL8 antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa
Western blot All lanes: RPL8 antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa
Western blot All lanes: RPL8 antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa

RPL8 Antibody

CSB-PA00595A0RB
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetRPL8
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Overview

  • Supplier
    Cusabio
  • Product Name
    RPL8 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6132
  • Target name
    RPL8
  • Target description
    ribosomal protein L8
  • Target synonyms
    60S ribosomal protein L8; L8; large ribosomal subunit protein uL2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62917
  • Protein Name
    60S ribosomal protein L8
  • Scientific Description
    This protein can be partially incorporated into E.coli polysomes in vivo, indicating it can replace the endogenous protein.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RPL8 using anti-RPL8 antibody (A06793-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL8 antigen affinity purified polyclonal antibody (Catalog # A06793-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL8 at approximately 34 kDa. The expected band size for RPL8 is at 34 kDa.
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