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WB analysis of various sample lysates using GTX54725 RPS19 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX54725 RPS19 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX54725 RPS19 antibody. Dilution : 1:1000 Loading : 25microg per lane

RPS19 antibody

GTX54725
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetRPS19
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Overview

  • Supplier
    GeneTex
  • Product Name
    RPS19 antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6223
  • Target name
    RPS19
  • Target description
    ribosomal protein S19
  • Target synonyms
    40S ribosomal protein S19; DBA; DBA1; eS19; LOH19CR1; loss of heterozygosity on chromosome 19, region 1; loss of heterozygosity, 19, chromosomal region 1; S19; small ribosomal subunit protein eS19
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP39019
  • Protein Name
    40S ribosomal protein S19
  • Scientific Description
    Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S19E family of ribosomal proteins. It is located in the cytoplasm. Mutations in this gene cause Diamond-Blackfan anemia (DBA), a constitutional erythroblastopenia characterized by absent or decreased erythroid precursors, in a subset of patients. This suggests a possible extra-ribosomal function for this gene in erythropoietic differentiation and proliferation, in addition to its ribosomal function. Higher expression levels of this gene in some primary colon carcinomas compared to matched normal colon tissues has been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RPS19 using anti-RPS19 antibody (A02343-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS19 antigen affinity purified polyclonal antibody (Catalog # A02343-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS19 at approximately 16 kDa. The expected band size for RPS19 is at 16 kDa.
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