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Lane 1: CRC Cells; Lane 2: MCF-7 Cells; Probed with _x000D_ RPS19 (1C1) Monoclonal Antibody (bsm-54242R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: CRC Cells; Lane 2: MCF-7 Cells; Probed with _x000D_ RPS19 (1C1) Monoclonal Antibody (bsm-54242R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: CRC Cells; Lane 2: MCF-7 Cells; Probed with _x000D_ RPS19 (1C1) Monoclonal Antibody (bsm-54242R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

RPS19 Recombinant Antibody

BSM-54242R
Bioss
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetRPS19
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Overview

  • Supplier
    Bioss
  • Product Name
    RPS19 Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IP(1-2ug), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID6223
  • Target name
    RPS19
  • Target description
    ribosomal protein S19
  • Target synonyms
    DBA, DBA1, LOH19CR1, S19, eS19, small ribosomal subunit protein eS19, 40S ribosomal protein S19, loss of heterozygosity on chromosome 19, region 1, loss of heterozygosity, 19, chromosomal region 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP39019
  • Protein Name
    Small ribosomal subunit protein eS19
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of RPS19 using anti-RPS19 antibody (A02343-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS19 antigen affinity purified polyclonal antibody (Catalog # A02343-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS19 at approximately 16 kDa. The expected band size for RPS19 is at 16 kDa.
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