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Western Blot analysis of NIH-3T3 cells using Ribosomal Protein S2 Polyclonal Antibody
Western Blot analysis of NIH-3T3 cells using Ribosomal Protein S2 Polyclonal Antibody
Western Blot analysis of NIH-3T3 cells using Ribosomal Protein S2 Polyclonal Antibody

RPS2 Antibody

CSB-PA003999
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRPS2
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Overview

  • Supplier
    Cusabio
  • Product Name
    RPS2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6187
  • Target name
    RPS2
  • Target description
    ribosomal protein S2
  • Target synonyms
    40S ribosomal protein S2; 40S ribosomal protein S4; LLREP3; OK/KNS-cl.6; protein LLRep3; S2; small ribosomal subunit protein uS5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15880
  • Protein Name
    40S ribosomal protein S2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RPS2 using anti-RPS2 antibody (A03548-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS2 antigen affinity purified polyclonal antibody (Catalog # A03548-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS2 at approximately 31 kDa. The expected band size for RPS2 is at 31 kDa.
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GeneTex
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