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RPS2 antibody [N2C3]

GTX114734
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetRPS2
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Overview

  • Supplier
    GeneTex
  • Product Name
    RPS2 antibody [N2C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6187
  • Target name
    RPS2
  • Target description
    ribosomal protein S2
  • Target synonyms
    40S ribosomal protein S2; 40S ribosomal protein S4; LLREP3; OK/KNS-cl.6; protein LLRep3; S2; small ribosomal subunit protein uS5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15880
  • Protein Name
    40S ribosomal protein S2
  • Scientific Description
    Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S5P family of ribosomal proteins. It is located in the cytoplasm. This gene shares sequence similarity with mouse LLRep3. It is co-transcribed with the small nucleolar RNA gene U64, which is located in its third intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Spatial organization and function of RNA molecules within phase-separated condensates are controlled by Dnd1.
    Read more
  • Simultaneous measurement of nascent transcriptome and translatome using 4-thiouridine metabolic RNA labeling and translating ribosome affinity purification.
    Read more
  • RACK1 Regulates Poxvirus Protein Synthesis Independently of Its Role in Ribosome-Based Stress Signaling.
    Read more
  • Negative charge in the RACK1 loop broadens the translational capacity of the human ribosome. Rollins MG et al., 2021 Sep 7, Cell Rep
    Read more
  • Dual Functional Roles of Molecular Beacon as a MicroRNA Detector and Inhibitor. Li WM et al., 2017 Mar 3, J Biol Chem
    Read more

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Figure 1. Western blot analysis of RPS2 using anti-RPS2 antibody (A03548-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS2 antigen affinity purified polyclonal antibody (Catalog # A03548-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS2 at approximately 31 kDa. The expected band size for RPS2 is at 31 kDa.
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