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Western Blot analysis of HuvEc cells using Ribosomal Protein S6 Polyclonal Antibody
Western Blot analysis of HuvEc cells using Ribosomal Protein S6 Polyclonal Antibody
Western Blot analysis of HuvEc cells using Ribosomal Protein S6 Polyclonal Antibody

RPS6 Antibody

CSB-PA004005
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRPS6
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Overview

  • Supplier
    Cusabio
  • Product Name
    RPS6 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6194
  • Target name
    RPS6
  • Target description
    ribosomal protein S6
  • Target synonyms
    S6, eS6, small ribosomal subunit protein eS6, 40S ribosomal protein S6, phosphoprotein NP33
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62753
  • Protein Name
    40S ribosomal protein S6
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RPS6 using anti-RPS6 antibody (A01567-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates, Lane 3: MCF-7 whole Cell lysates, Lane 4: A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS6 antigen affinity purified polyclonal antibody (Catalog # A01567-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS6 at approximately 32KD. The expected band size for RPS6 is at 29KD.
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