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RPS6KA2 / RSK3 Antibody

LS-C813756
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetRPS6KA2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RPS6KA2 / RSK3 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000), WB (1:5000 - 1:10000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6196
  • Target name
    RPS6KA2
  • Target description
    ribosomal protein S6 kinase A2
  • Target synonyms
    HU-2, MAPKAPK1C, RSK, RSK3, S6K-alpha, S6K-alpha2, p90-RSK3, p90RSK2, pp90RSK3, ribosomal protein S6 kinase alpha-2, MAP kinase-activated protein kinase 1c, MAPK-activated protein kinase 1c, mitogen-activated protein kinase-activated protein kinase 1C, ribosomal S6 kinase 3, ribosomal protein S6 kinase, 90kDa, polypeptide 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RSK3 using anti-RSK3 antibody (A06142-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysate, Lane 3: human PC-3 whole cell lysate, Lane 4: human A431 whole cell lysate, Lane 5: human K562 whole cell lysate, Lane 6: human PANC-1 whole cell lysate, Lane 7: rat testis tissue lysates, Lane 8: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RSK3 antigen affinity purified polyclonal antibody (Catalog # A06142-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RSK3 at approximately 90-100KD. The expected band size for RSK3 is at 83KD.
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Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with _x000D_ p90RSK (Ser380) Polyclonal Antibody (bs-3362R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
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