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S6K1 Antibody(Rabbit)

HY-P81209
MedChem Express
TargetRPS6KB1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    S6K1 Antibody(Rabbit)
  • Delivery Days Customer
    14
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID6198
  • Target name
    RPS6KB1
  • Target description
    ribosomal protein S6 kinase B1
  • Target synonyms
    PS6K, S6K, S6K-beta-1, S6K1, STK14A, p70 S6KA, p70(S6K)-alpha, p70-S6K, p70-alpha, ribosomal protein S6 kinase beta-1, ribosomal protein S6 kinase I, ribosomal protein S6 kinase, 70kDa, polypeptide 1, serine/threonine kinase 14 alpha, serine/threonine-protein kinase 14A
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    S6K1 Antibody is an unconjugated, approximately 59 kDa, rabbit-derived, anti-S6K1 monoclonal antibody. S6K1 Antibody can be used for: WB, ICC/IF, IP expriments in human, rat, mouse background without labeling.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of S6K1/RPS6KB1 using anti-S6K1/RPS6KB1 antibody (A01475-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT4 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human placenta tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S6K1/RPS6KB1 antigen affinity purified polyclonal antibody (Catalog # A01475-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S6K1/RPS6KB1 at approximately 59 kDa. The expected band size for S6K1/RPS6KB1 is at 59 kDa.
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References
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