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Confocal immunofluorescence analysis of HeLa cells using RRBP1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of HeLa cells using RRBP1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of HeLa cells using RRBP1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

RRBP1 Polyclonal Antibody

E-AB-63671
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    RRBP1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes a ribosome-binding protein of the endoplasmic reticulum (ER) membrane. Studies suggest that this gene plays a role in ER proliferation, secretory pathways and secretory cell differentiation, and mediation of ER-microtubule interactions. Alternative splicing has been observed and protein isoforms are characterized by regions of N-terminal decapeptide and C-terminal heptad repeats. Splicing of the tandem repeats results in variations in ribosome-binding affinity and secretory function. The full-length nature of variants which differ in repeat length has not been determined. Pseudogenes of this gene have been identified on chromosomes 3 and 7, and RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome, the result of a mutation in a nearby gene on chromosome 20p12.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RRBP1 using anti-RRBP1 antibody (A07074-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRBP1 antigen affinity purified polyclonal antibody (Catalog # A07074-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRBP1 at approximately 200KD. The expected band size for RRBP1 is at 152KD.
Product group Antibodies
Boster Bio
Anti-RRBP1 Antibody Picoband(r)A07074-1-CARRIER-FREE
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ReactivityHuman, Mouse, Rat
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ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rat
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Immunohistochemistry analysis in human placenta and skeletal muscle tissues using Anti-RRBP1 antibody. Corresponding RRBP1 RNA-seq data are presented for the same tissues.
Product group Antibodies
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The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA083779(RRBP1 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)
Product group Antibodies
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Ribosome binding protein 1 antibody validation by siRNA knock-down. Upperpanel: Ribosome binding protein 1 antibody GTX101844 Lower panel: Vinculin antibody (GTX109749) A. 30 μg HepG2 whole cell lysate/extract B. 30 μg whole cell lysate/extract of RRBP1 siRNA#1-transfected HepG2 cells C. 30 μg whole cell lysate/extract of RRBP1 siRNA#2-transfected HepG2 cells 5% SDS-PAGE Ribosome binding protein 1 antibody (GTX101844) dilution: 1:500 Vinculin antibody (GTX109749) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
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Ribosome binding protein 1 antibody [C3], C-termGTX101844
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ReactivityHuman, Mouse
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