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RRP7A Rabbit pAb

A9617
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRRP7A
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Overview

  • Supplier
    ABclonal
  • Product Name
    RRP7A Rabbit pAb
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27341
  • Target name
    RRP7A
  • Target description
    ribosomal RNA processing 7 homolog A
  • Target synonyms
    BK126B4.3, CGI-96, MCPH28, Rrp7, ribosomal RNA-processing protein 7 homolog A, CTA-126B4.5, gastric cancer antigen Zg14
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y3A4
  • Protein Name
    Ribosomal RNA-processing protein 7 homolog A
  • Reactivity
    Human, Mouse, Rat
  • Reactivity Supplier Note
    Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RRP7A using anti-RRP7A antibody (A15583-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A341 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRP7A antigen affinity purified polyclonal antibody (Catalog # A15583-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRP7A at approximately 37 kDa. The expected band size for RRP7A is at 32 kDa.
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