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Western Blot analysis of HuvEc cells using RRS1 Polyclonal Antibody
Western Blot analysis of HuvEc cells using RRS1 Polyclonal Antibody
Western Blot analysis of HuvEc cells using RRS1 Polyclonal Antibody

RRS1 Antibody

CSB-PA004028
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRRS1
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Overview

  • Supplier
    Cusabio
  • Product Name
    RRS1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23212
  • Target name
    RRS1
  • Target description
    ribosome biogenesis regulator 1 homolog
  • Target synonyms
    homolog of yeast ribosome biogenesis regulator; homolog of yeast ribosome biogenesis regulatory protein RRS1; ribosome biogenesis regulator homolog; ribosome biogenesis regulatory protein homolog; ribosome biogenesis regulatory protein RRS1 homolog; RRS1 ribosome biogenesis regulator homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15050
  • Protein Name
    Ribosome biogenesis regulatory protein homolog
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RRS1 using anti-RRS1 antibody (A04418-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human MDA-MB-453 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: rat RH35 whole cell lysates, Lane 9: mouse NIH/3T3 whole cell lysates, Lane 10: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRS1 antigen affinity purified polyclonal antibody (Catalog # A04418-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRS1 at approximately 39 kDa. The expected band size for RRS1 is at 39 kDa.
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