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WB analysis of HUVEC and 293 cell lysates using GTX87140 RRS1 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of HUVEC and 293 cell lysates using GTX87140 RRS1 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of HUVEC and 293 cell lysates using GTX87140 RRS1 antibody. The lane on the right is blocked with the synthesized peptide.

RRS1 antibody

GTX87140
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetRRS1
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Overview

  • Supplier
    GeneTex
  • Product Name
    RRS1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23212
  • Target name
    RRS1
  • Target description
    regulator of ribosome synthesis 1
  • Target synonyms
    ribosome biogenesis regulatory protein homolog, RRS1 ribosome biogenesis regulator homolog, homolog of yeast ribosome biogenesis regulator, homolog of yeast ribosome biogenesis regulatory protein RRS1, ribosome biogenesis regulator 1 homolog, ribosome biogenesis regulator homolog, ribosome biogenesis regulatory protein RRS1 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15050
  • Protein Name
    Ribosome biogenesis regulatory protein homolog
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RRS1 using anti-RRS1 antibody (A04418-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human MDA-MB-453 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: rat RH35 whole cell lysates, Lane 9: mouse NIH/3T3 whole cell lysates, Lane 10: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRS1 antigen affinity purified polyclonal antibody (Catalog # A04418-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRS1 at approximately 39 kDa. The expected band size for RRS1 is at 39 kDa.
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