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WB analysis of human heart tissue using GTX47378 RTCD1 antibody at 0.2-1microg/ml.
WB analysis of human heart tissue using GTX47378 RTCD1 antibody at 0.2-1microg/ml.
WB analysis of human heart tissue using GTX47378 RTCD1 antibody at 0.2-1microg/ml.

RTCD1 antibody, N-term

GTX47378
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetRTCA
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Overview

  • Supplier
    GeneTex
  • Product Name
    RTCD1 antibody, N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8634
  • Target name
    RTCA
  • Target description
    RNA 3'-terminal phosphate cyclase
  • Target synonyms
    RNA 3'-terminal phosphate cyclase; RNA terminal phosphate cyclase domain 1; RNA terminal phosphate cyclase domain-containing protein 1; RPC; RTC domain-containing protein 1; RTC1; RTCD1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00442
  • Protein Name
    RNA 3'-terminal phosphate cyclase
  • Scientific Description
    This gene encodes a member of the RNA 3-phosphate cyclase family. The encoded protein plays a role in RNA metabolism by catalyzing the ATP-dependent conversion of the 3-phosphate of RNA substrates to a 2,3-cyclic phosphodiester. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RTCD1/RTCA using anti-RTCD1/RTCA antibody (A07570-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTCD1/RTCA antigen affinity purified polyclonal antibody (Catalog # A07570-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTCD1/RTCA at approximately 39KD. The expected band size for RTCD1/RTCA is at 39KD.
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