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Western Blot Positive WB detected in: HEK293 whole cell lysate, Ntera-2 whole cell lysate All lanes: RUVBL1 antibody at 3.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51, 43 kDa Observed band size: 51 kDa
Western Blot Positive WB detected in: HEK293 whole cell lysate, Ntera-2 whole cell lysate All lanes: RUVBL1 antibody at 3.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51, 43 kDa Observed band size: 51 kDa
Western Blot Positive WB detected in: HEK293 whole cell lysate, Ntera-2 whole cell lysate All lanes: RUVBL1 antibody at 3.2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51, 43 kDa Observed band size: 51 kDa

RUVBL1 Antibody

CSB-PA896483LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRUVBL1
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Overview

  • Supplier
    Cusabio
  • Product Name
    RUVBL1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8607
  • Target name
    RUVBL1
  • Target description
    RuvB like AAA ATPase 1
  • Target synonyms
    ECP-54, ECP54, INO80H, NMP 238, NMP238, PONTIN, Pontin52, RVB1, TIH1, TIP49, TIP49A, ruvB-like 1, 49 kDa TATA box-binding protein-interacting protein, 49 kDa TBP-interacting protein, 54 kDa erythrocyte cytosolic protein, INO80 complex subunit H, RuvB (E coli homolog)-like 1, RuvB-like AAA ATPase, TAP54-alpha, TATA binding protein interacting protein 49 kDa, TIP60-associated protein 54-alpha, epididymis secretory sperm binding protein, nuclear matrix protein 238, pontin 52
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y265
  • Protein Name
    RuvB-like 1
  • Scientific Description
    Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3 to 5) activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TIP49A/RUVBL1 using anti-TIP49A/RUVBL1 antibody (A02049-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human Daudi whole cell lysates, Lane 7: human MOLT-4 whole cell lysates, Lane 8: human HEL whole cell lysates, Lane 9: rat testis tissue lysates, Lane 10: rat C6 whole cell lysates, Lane 11: mouse testis tissue lysates, Lane 12: mouse NIH/3T3 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIP49A/RUVBL1 antigen affinity purified polyclonal antibody (Catalog # A02049-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIP49A/RUVBL1 at approximately 54 kDa. The expected band size for TIP49A/RUVBL1 is at 50 kDa.
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