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Western Blot analysis of Jurkat cells using S-100A10 Polyclonal Antibody
Western Blot analysis of Jurkat cells using S-100A10 Polyclonal Antibody
Western Blot analysis of Jurkat cells using S-100A10 Polyclonal Antibody

S100A10 Antibody

CSB-PA004043
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetS100A10
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Overview

  • Supplier
    Cusabio
  • Product Name
    S100A10 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6281
  • Target name
    S100A10
  • Target description
    S100 calcium binding protein A10
  • Target synonyms
    42C, ANX2L, ANX2LG, CAL1L, CLP11, Ca[1], GP11, P11, p10, protein S100-A10, S100 calcium binding protein A10 (annexin II ligand, calpactin I, light polypeptide (p11)), annexin II ligand, calpactin I, light polypeptide, annexin II tetramer (AIIt) p11 subunit, calpactin I light chain, calpactin-1 light chain, cellular ligand of annexin II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP60903
  • Protein Name
    Protein S100-A10
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of S100A10 using anti-S100A10 antibody (A02787-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: monkey lung tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A10 antigen affinity purified polyclonal antibody (Catalog # A02787-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A10 at approximately 11 kDa. The expected band size for S100A10 is at 11 kDa.
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Immunohistochemistry analysis in human lung and skeletal muscle tissues using HPA003340 antibody. Corresponding S100A10 RNA-seq data are presented for the same tissues.
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