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Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with S100A11 Polyclonal Antibody, Unconjugated (bs-55189R) at 1:100 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with S100A11 Polyclonal Antibody, Unconjugated (bs-55189R) at 1:100 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with S100A11 Polyclonal Antibody, Unconjugated (bs-55189R) at 1:100 overnight at 4°C, DAB staining.

S100A11 Polyclonal Antibody

BS-55189R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetS100A11
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Overview

  • Supplier
    Bioss
  • Product Name
    S100A11 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:500-2000), ICC/IF(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6282
  • Target name
    S100A11
  • Target description
    S100 calcium binding protein A11
  • Target synonyms
    HEL-S-43, MLN70, S100C, protein S100-A11, MLN 70, calgizzarin, epididymis secretory protein Li 43, metastatic lymph node gene 70 protein, protein S100-C
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31949
  • Protein Name
    Protein S100-A11
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

Datasheet

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Figure 1. Western blot analysis of S100A11 using anti-S100A11 antibody (PB9818). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A11 antigen affinity purified polyclonal antibody (Catalog # PB9818) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A11 at approximately 15 kDa. The expected band size for S100A11 is at 15 kDa.
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