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Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with S100A4 Polyclonal Antibody, Unconjugated (bs-3759R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with S100A4 Polyclonal Antibody, Unconjugated (bs-3759R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with S100A4 Polyclonal Antibody, Unconjugated (bs-3759R) at 1:200 overnight at 4°C, DAB staining.

S100A4 Polyclonal Antibody

BS-3759R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat
TargetS100A4
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Overview

  • Supplier
    Bioss
  • Product Name
    S100A4 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6275
  • Target name
    S100A4
  • Target description
    S100 calcium binding protein A4
  • Target synonyms
    18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98, protein S100-A4, S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog), calcium placental protein, fibroblast-specific protein-1, leukemia multidrug resistance associated protein, malignant transformation suppression 1, metastasin 1, murine placental homolog, placental calcium-binding protein, protein Mts1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP26447
  • Protein Name
    Protein S100-A4
  • Reactivity
    Bovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • Ginkgo biloba extract may alleviate viral myocarditis by suppression of S100A4 and MMP-3. Wang W et al., 2019 Dec, J Med Virol
    Read this paper
  • TGFbeta1 is essential for MSCs-CAFs differentiation and promotes HCT116 cells migration and invasion via JAK/STAT3 signaling. Tan HX et al., 2019, Onco Targets Ther
    Read this paper
  • Zinc oxide nanoparticles ameliorate collagen lattice contraction in human tenon fibroblasts. Yin X et al., 2019 Jul 15, Arch Biochem Biophys
    Read this paper
  • Homemade-device-induced negative pressure promotes wound healing more efficiently than VSD-induced positive pressure by regulating inflammation, proliferation and remodeling. Liu J et al., 2017 Apr, Int J Mol Med
    Read this paper

Datasheet

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Figure 1. Western blot analysis of S100A4 using anti-S100A4 antibody (A01217-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A4 antigen affinity purified polyclonal antibody (Catalog # A01217-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A4 at approximately 10-12KD. The expected band size for S100A4 is at 10-12KD.
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