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S100A4 Polyclonal Antibody

CAC13919
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetS100A4
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Overview

  • Supplier
    Biomatik
  • Product Name
    S100A4 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6275
  • Target name
    S100A4
  • Target description
    S100 calcium binding protein A4
  • Target synonyms
    18A2; 42A; calcium placental protein; CAPL; fibroblast-specific protein-1; FSP1; leukemia multidrug resistance associated protein; malignant transformation suppression 1; metastasin 1; MTS1; murine placental homolog; P9KA; PEL98; placental calcium-binding protein; protein Mts1; protein S100-A4; S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP26447
  • Protein Name
    Protein S100-A4
  • Scientific Description
    The S100A4 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of S100A4 using anti-S100A4 antibody (A01217-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A4 antigen affinity purified polyclonal antibody (Catalog # A01217-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A4 at approximately 10-12KD. The expected band size for S100A4 is at 10-12KD.
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Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with S100A4 Polyclonal Antibody, Unconjugated (bs-3759R) at 1:200 overnight at 4°C, DAB staining.
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