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S100A7 / Psoriasin Antibody

LS-C408380
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetS100A7
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    S100A7 / Psoriasin Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 11kDa, while the observed MW by Western blot was Refer to Figures.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 11kDa, while the observed MW by Western blot was Refer to Figures.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.14 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6278
  • Target name
    S100A7
  • Target description
    S100 calcium binding protein A7
  • Target synonyms
    protein S100-A7; PSOR1; psoriasin 1; S100A7c
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Psoriasin using anti-Psoriasin antibody (A02369-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela cell lysates, Lane 3: rat spleen tissue lysates, Lane 4: rat RH35 cell lysates, Lane 5: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Psoriasin antigen affinity purified polyclonal antibody (Catalog # A02369-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Psoriasin at approximately 17KD. The expected band size for Psoriasin is at 11KD.
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