More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/S100A9 / MRP14 Antibody (aa2-113)

S100A9 / MRP14 Antibody (aa2-113)

LS-C407890
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityMouse, Rat
TargetS100A9
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    S100A9 / MRP14 Antibody (aa2-113)
  • Delivery Days Customer
    23
  • Application Supplier Note
    IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA (0.1 - 0.5 µg/ml), IHC, IHC-P (0.5 - 1 µg/ml), WB (0.1 - 0.5 µg/ml) IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6280
  • Target name
    S100A9
  • Target description
    S100 calcium binding protein A9
  • Target synonyms
    60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14, S100-A9, protein S100-A9, MRP-14, calgranulin B, calprotectin L1H subunit, leukocyte L1 complex heavy chain, migration inhibitory factor-related protein 14
  • Host
    Rabbit
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

IHC image of CSB-PA020642HA01HU diluted at 1:400 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
S100A9 AntibodyCSB-PA020642HA01HU
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetS100A9
  • SizePrice
Product group Antibodies
Assay Genie
S100A9 AntibodyCAB9842
ReactivityHuman
TargetS100A9
  • SizePrice
Immunohistochemistry analysis in human bone marrow and kidney tissues using AMAb91690 antibody. Corresponding S100A9 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-S100A9 AntibodyAMAB91690
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetS100A9
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-S100A9 [IID4B10]Ab02468-1.1
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetS100A9
  • SizePrice
Product group Antibodies
Antibodies.com
Anti-Calprotectin AntibodyA82044
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
S100A9 / MRP14 AntibodyLS-C831465
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetS100A9
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-S100A9EB09146
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetS100A9
  • SizePrice
Product group Antibodies
Biomatik
S100A9 Polyclonal AntibodyCAC07212
ApplicationsImmunoFluorescence, ELISA
TargetS100A9
  • SizePrice
Figure 1. Western blot analysis of S100A9 using anti-S100A9 antibody (PB9677). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: A431 Whole Cell Lysate, Lane 2: MCF-7 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A9 antigen affinity purified polyclonal antibody (Catalog # PB9677) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A9 at approximately 13 kDa. The expected band size for S100A9 is at 13 kDa.
Product group Antibodies
Boster Bio
Anti-S100A9 Antibody Picoband(r)PB9677-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetS100A9
  • SizePrice
S100A9 antibody detects S100A9 protein at secreted on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. S100A9 antibody (GTX129575) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Product group Antibodies
GeneTex
S100A9 antibodyGTX129575
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetS100A9
  • SizePrice