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SA1 antibody

GTX129912
GeneTex
ApplicationsImmunoPrecipitation, Western Blot
DownloadDatasheet
Product group Antibodies
TargetSTAG1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SA1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.53 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10274
  • Target name
    STAG1
  • Target description
    stromal antigen 1
  • Target synonyms
    cohesin subunit SA-1; MRD47; nuclear protein stromal antigen 1; SA1; SCC3 homolog 1; SCC3A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WVM7
  • Protein Name
    Cohesin subunit SA-1
  • Scientific Description
    This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Precision RNAi using synthetic shRNAmir target sites.
    Read more
  • Paralogous synthetic lethality underlies genetic dependencies of the cancer-mutated gene STAG2. Bailey ML et al., 2021 Nov, Life Sci Alliance
    Read more
  • STAG1 vulnerabilities for exploiting cohesin synthetic lethality in STAG2-deficient cancers. van der Lelij P et al., 2020 Jul, Life Sci Alliance
    Read more
  • Synthetic lethality between the cohesin subunits STAG1 and STAG2 in diverse cancer contexts. van der Lelij P et al., 2017 Jul 10, Elife
    Read more

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Figure 1. Western blot analysis of SA1/STAG1 using anti-SA1/STAG1 antibody (A06471-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SA1/STAG1 antigen affinity purified polyclonal antibody (Catalog # A06471-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SA1/STAG1 at approximately 155 kDa. The expected band size for SA1/STAG1 is at 144 kDa.
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