Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).
![Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) (1 μg/mL). Human IgG antibody (HRP) (1:200000) was used to detect bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635866/GTX635866_44354_20210910_ELISA_Indirect_w_23061202_468.webp "Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) (1 μg/mL). Human IgG antibody (HRP) (1:200000) was used to detect bound primary antibody.")
![Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) (1 μg/mL) Anti-Human IgG antibody (HRP) was diluted at 1:200000 and used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635866/GTX635866_44613_20220311_ELISA_PAIR_w_23061202_781.webp "Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) (1 μg/mL) Anti-Human IgG antibody (HRP) was diluted at 1:200000 and used to detect the primary antibody.")
Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).
SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU]
GTX635866
ApplicationsELISA, Neutralisation/Blocking
Product group Antibodies
ReactivityVirus
TargetS
Overview
- SupplierGeneTex
- Product NameSARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU]
- Delivery Days Customer9
- Application Supplier Note*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsELISA, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1003-HU
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID43740568
- Target nameS
- Target descriptionsurface glycoprotein
- Target synonymsGU280_gp02, spike glycoprotein, surface glycoprotein
- HostHuman
- IsotypeIgG
- Protein IDP0DTC2
- Protein NameSpike glycoprotein
- ReactivityVirus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Cheedarla N, Verkerke HP, Potlapalli S, et al. Rapid, high throughput, automated detection of SARS-CoV-2 neutralizing antibodies against Wuhan-WT, delta and omicron BA1, BA2 spike trimers. iScience. 2023,26(11):108256. doi: 10.1016/j.isci.2023.108256Read this paper
Datasheet
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