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Goat anti-SART1 Antibody

ORB18886
Biorbyt
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityBovine, Human, Mouse, Rat
TargetSART1
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Goat anti-SART1 Antibody
  • Delivery Days Customer
    16
  • Application Supplier Note
    ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 125kDa band observed in HeLa lysates (consistent with Vertegaal et al, below, calculated MW of 90.3 kDa according to NP_005137). Recommended concentration: 0.03-0.1 microg/ml.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA: 1:64000, WB: 0.03-0.1 μg/ml ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9092
  • Target name
    SART1
  • Target description
    spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP
  • Target synonyms
    Ara1, HAF, HOMS1, SART1259, SNRNP110, Snu66, U4/U6.U5 tri-snRNP-associated protein 1, IgE autoantigen, SART-1, SART1(259) protein, SART1(800) protein, SART1, U4/U6.U5 tri-snRNP-associated protein 1, SNU66 homolog, U4/U6.U5 tri-snRNP-associated 110 kDa protein, hSART-1, hSnu66, hypoxia associated factor, small nuclear ribonucleoprotein 110kDa (U4/U6.U5), squamous cell carcinoma antigen recognised by T cells, squamous cell carcinoma antigen recognized by T-cells 1
  • Host
    Goat
  • Scientific Description
    Goat polyclonal antibody to SART1
  • Reactivity
    Bovine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SART1 using anti-SART1 antibody (A05414-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SART1 antigen affinity purified polyclonal antibody (Catalog # A05414-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SART1 at approximately 110 kDa. The expected band size for SART1 is at 90 kDa.
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