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Western blot analysis of extracts of various cell lines using SART3 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using SART3 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using SART3 Polyclonal Antibody at dilution of 1:3000.

SART3 Polyclonal Antibody

E-AB-63630
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    SART3 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SART3 using anti-SART3 antibody (A03973-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SART3 antigen affinity purified polyclonal antibody (Catalog # A03973-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SART3 at approximately 130 kDa. The expected band size for SART3 is at 110 kDa.
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Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells.
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