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ProductsBack/SCA2 / LY6E Antibody (aa46-56)

SCA2 / LY6E Antibody (aa46-56)

LS-C113031
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetLY6E
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SCA2 / LY6E Antibody (aa46-56)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary experiments in Mouse Brain, Kidney, Heart and Spleen lysates gave no specific signal but low background (at antibody concentration up to 1 ug/ml).. IF (10 µg/ml), IHC, Peptide-ELISA (1:16000) Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary experiments in Mouse Brain, Kidney, Heart and Spleen lysates gave no specific signal but low background (at antibody concentration up to 1 ug/ml).
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4061
  • Target name
    LY6E
  • Target description
    lymphocyte antigen 6 family member E
  • Target synonyms
    RIG-E, RIGE, SCA-2, SCA2, TSA-1, lymphocyte antigen 6E, ly-6E, lymphocyte antigen 6 complex, locus E, retinoic acid induced gene E, retinoic acid-induced gene E protein, stem cell antigen 2, thymic shared antigen 1
  • Host
    Goat
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LY6E/SCA-2 using anti-LY6E/SCA-2 antibody (A09496-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LY6E/SCA-2 antigen affinity purified polyclonal antibody (Catalog # A09496-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LY6E/SCA-2 at approximately 14 kDa. The expected band size for LY6E/SCA-2 is at 14 kDa.
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