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SCARA3 Antibody

PACO36438
Assay Genie
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSCARA3
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Overview

  • Supplier
    Assay Genie
  • Product Name
    SCARA3 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID51435
  • Target name
    SCARA3
  • Target description
    scavenger receptor class A member 3
  • Target synonyms
    APC7, CSR, CSR1, MSLR1, MSRL1, scavenger receptor class A member 3, cellular stress response gene protein, cellular stress response protein, macrophage scavenger receptor-like 1
  • Host
    Rabbit
  • Protein IDQ6AZY7
  • Protein Name
    Scavenger receptor class A member 3
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human, Mouse
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

Datasheet

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MSDS

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Western blot All lanes: SCARA3 antibody at 0.8microg/ml Lane 1: Mouse liver tissue Lane 2: Mouse kidney tissue Lane 3: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 66, 53 kDa Observed band size: 66 kDa
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Figure 1. Western blot analysis of SCARA3 using anti-SCARA3 antibody (A11048-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCARA3 antigen affinity purified polyclonal antibody (Catalog # A11048-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCARA3 at approximately 65 kDa. The expected band size for SCARA3 is at 65 kDa.
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