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Western Blot Positive WB detected in: HepG2 whole cell lysate, Mouse liver tissue All lanes: SCARB2 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55, 38 kDa Observed band size: 55 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Mouse liver tissue All lanes: SCARB2 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55, 38 kDa Observed band size: 55 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Mouse liver tissue All lanes: SCARB2 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55, 38 kDa Observed band size: 55 kDa

SCARB2 Antibody

CSB-PA619859LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSCARB2
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Overview

  • Supplier
    Cusabio
  • Product Name
    SCARB2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID950
  • Target name
    SCARB2
  • Target description
    scavenger receptor class B member 2
  • Target synonyms
    AMRF, CD36L2, EPM4, HLGP85, LGP85, LIMP-2, LIMPII, SR-BII, lysosome membrane protein 2, 85 kDa lysosomal membrane sialoglycoprotein, 85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2, CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2 (lysosomal integral membrane protein II), CD36 antigen-like 2, LIMP II, lysosome membrane protein II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14108
  • Protein Name
    Lysosome membrane protein 2
  • Scientific Description
    Acts as a lysosomal receptor for glucosylceramidase (GBA) targeting.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of Scavenging Receptor SRB2/SCARB2 using anti-Scavenging Receptor SRB2/SCARB2 antibody (A05090-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Scavenging Receptor SRB2/SCARB2 antigen affinity purified polyclonal antibody (Catalog # A05090-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Scavenging Receptor SRB2/SCARB2 at approximately 80 kDa. The expected band size for Scavenging Receptor SRB2/SCARB2 is at 54 kDa.
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