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ProductsBack/Scavenger Receptor BII Antibody
Antigen: bs-7545P, 2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \nTMB staining\nRead the data in Microplate Reader by 450nm. \n
Antigen: bs-7545P, 2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \nTMB staining\nRead the data in Microplate Reader by 450nm. \n
Antigen: bs-7545P, 2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \nTMB staining\nRead the data in Microplate Reader by 450nm. \n

Scavenger Receptor BII Antibody

BS-7545R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSCARB2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Scavenger Receptor BII Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID950
  • Target name
    SCARB2
  • Target description
    scavenger receptor class B member 2
  • Target synonyms
    85 kDa lysosomal membrane sialoglycoprotein; 85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2; AMRF; CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2 (lysosomal integral membrane protein II); CD36 antigen-like 2; CD36L2; EPM4; HLGP85; LGP85; LIMP II; LIMP-2; LIMPII; lysosome membrane protein 2; lysosome membrane protein II; SR-BII
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14108
  • Protein Name
    Lysosome membrane protein 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Scavenging Receptor SRB2/SCARB2 using anti-Scavenging Receptor SRB2/SCARB2 antibody (A05090-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Scavenging Receptor SRB2/SCARB2 antigen affinity purified polyclonal antibody (Catalog # A05090-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Scavenging Receptor SRB2/SCARB2 at approximately 80 kDa. The expected band size for Scavenging Receptor SRB2/SCARB2 is at 54 kDa.
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