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SCD antibody

GTX04646
GeneTex
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
DownloadDatasheet
Product group Antibodies
TargetSCD
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Overview

  • Supplier
    GeneTex
  • Product Name
    SCD antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.25-0.5 microg/mL. FACS: 1-3 microg/106 cells. ELISA: 0.1-0.5 microg/mL. IHC: 2-5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6319
  • Target name
    SCD
  • Target description
    stearoyl-CoA desaturase
  • Target synonyms
    acyl-CoA desaturase; delta(9)-desaturase; FADS5; fatty acid desaturase; hSCD1; MSTP008; predicted protein of HQ0998; SCD1; SCDOS; stearoyl-CoA desaturase; stearoyl-CoA desaturase (delta-9-desaturase); stearoyl-CoA desaturase opposite strand
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00767
  • Protein Name
    Acyl-CoA desaturase
  • Scientific Description
    This gene encodes an enzyme involved in fatty acid biosynthesis, primarily the synthesis of oleic acid. The protein belongs to the fatty acid desaturase family and is an integral membrane protein located in the endoplasmic reticulum. Transcripts of approximately 3.9 and 5.2 kb, differing only by alternative polyadenlyation signals, have been detected. A gene encoding a similar enzyme is located on chromosome 4 and a pseudogene of this gene is located on chromosome 17. [provided by RefSeq, Sep 2015]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SCD using anti-SCD antibody (A00588-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCD antigen affinity purified polyclonal antibody (Catalog # A00588-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCD at approximately 37,42 kDa. The expected band size for SCD is at 42 kDa.
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