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SCIN Antibody (aa281-585)

LS-C186278
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
TargetSCIN
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SCIN Antibody (aa281-585)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    ICC (1:100 - 1:1000), WB (1:500 - 1:3000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID85477
  • Target name
    SCIN
  • Target description
    scinderin
  • Target synonyms
    adseverin
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SCIN using anti-SCIN antibody (A07463). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates, Lane 4: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCIN antigen affinity purified polyclonal antibody (Catalog # A07463) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCIN at approximately 80 kDa. The expected band size for SCIN is at 80 kDa.
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