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Western Blot Positive WB detected in: Mouse stomach tissue All lanes: SCIN antibody at 3.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81, 66, 53 kDa Observed band size: 81 kDa
Western Blot Positive WB detected in: Mouse stomach tissue All lanes: SCIN antibody at 3.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81, 66, 53 kDa Observed band size: 81 kDa
Western Blot Positive WB detected in: Mouse stomach tissue All lanes: SCIN antibody at 3.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 81, 66, 53 kDa Observed band size: 81 kDa

SCIN Antibody

CSB-PA020823LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetSCIN
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Overview

  • Supplier
    Cusabio
  • Product Name
    SCIN Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID85477
  • Target name
    SCIN
  • Target description
    scinderin
  • Target synonyms
    adseverin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y6U3
  • Protein Name
    Adseverin
  • Scientific Description
    Ca(2+)-dependent actin filament-severing protein that is presumed to have a regulatory function in exocytosis by affecting the organization of the microfilament network underneath the plasma membrane. In vitro, also has barbed end capping and nucleating activities in the presence of Ca(2+). Regulates chondrocyte proliferation and differentiation. MAP kinases p38 and ERK1/2 mediate the adseverin-induced accelerated differentiation of non-hypertrophic chondrocytes (By similarity).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SCIN using anti-SCIN antibody (A07463). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates, Lane 4: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCIN antigen affinity purified polyclonal antibody (Catalog # A07463) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCIN at approximately 80 kDa. The expected band size for SCIN is at 80 kDa.
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