Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Sclerostin) Polyclonal Antibody, Unconjugated (bs-10200R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Sclerostin) Polyclonal Antibody, Unconjugated (bs-10200R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sclerostin Polyclonal Antibody
BS-10200R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetSOST
Overview
- SupplierBioss
- Product NameSclerostin Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID50964
- Target nameSOST
- Target descriptionsclerostin
- Target synonymsCDD, DAND6, SOST1, VBCH, sclerostin
- HostRabbit
- IsotypeIgG
- Protein IDQ9BQB4
- Protein NameSclerostin
- ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
- Storage Instruction-20°C
- UNSPSC12352203
References
- Surowiec RK, Battle LF, Ward FS, et al. A xenograft model to evaluate the bone forming effects of sclerostin antibody in human bone derived from pediatric osteogenesis imperfecta patients. Bone. 2020,130:115118. doi: 10.1016/j.bone.2019.115118Read this paper
- Diao X, Li Z, An B, et al. The Microdamage and Expression of Sclerostin in Peri-implant Bone under One-time Shock Force Generated by Impact. Sci Rep. 2017,7(1):6508. doi: 10.1038/s41598-017-06867-9Read this paper
Datasheet
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