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SCO1 Polyclonal Antibody

RD84043A
Reddot Biotech
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSCO1
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    SCO1 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6341
  • Target name
    SCO1
  • Target description
    synthesis of cytochrome C oxidase 1
  • Target synonyms
    MC4DN4, SCOD1, protein SCO1 homolog, mitochondrial, SCO cytochrome c oxidase assembly protein 1, SCO cytochrome oxidase deficient homolog 1, SCO1, cytochrome c oxidase assembly protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75880
  • Protein Name
    Protein SCO1 homolog, mitochondrial
  • Scientific Description
    Mammalian cytochrome c oxidase (COX) catalyzes the transfer of reducing equivalents from cytochrome c to molecular oxygen and pumps protons across the inner mitochondrial membrane. In yeast, 2 related COX assembly genes, SCO1 and SCO2 (synthesis of cytochrome c oxidase), enable subunits 1 and 2 to be incorporated into the holoprotein. This gene is the human homolog to the yeast SCO1 gene. - This is a SCO1 Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of SCO1 using anti-SCO1 antibody (A02934-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCO1 antigen affinity purified polyclonal antibody (Catalog # A02934-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCO1 at approximately 30 kDa. The expected band size for SCO1 is at 34 kDa.
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