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SDF1 antibody

GTX10395
GeneTex
ApplicationsWestern Blot, ELISA, Neutralisation/Blocking
Product group Antibodies
TargetCXCL12
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Overview

  • Supplier
    GeneTex
  • Product Name
    SDF1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.2 microg/ml. ELISA: 0.5-1 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6387
  • Target name
    CXCL12
  • Target description
    C-X-C motif chemokine ligand 12
  • Target synonyms
    IRH, PBSF, SCYB12, SDF1, TLSF, TPAR1, stromal cell-derived factor 1, chemokine (C-X-C motif) ligand 12, intercrine reduced in hepatomas, pre-B cell growth-stimulating factor
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDP48061
  • Protein Name
    Stromal cell-derived factor 1
  • Scientific Description
    SDF-1 is a cytokine implicated in modulating cell migration.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CXCL12 using anti-CXCL12 antibody (A00053-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL12 antigen affinity purified polyclonal antibody (Catalog # A00053-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL12 at approximately 11 kDa. The expected band size for CXCL12 is at 11 kDa.
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