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Western blot analysis of SRC using anti-SRC antibody (MP00107) in A431 cell lysate treated with pervanadate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRC antigen affinity purified polyclonal antibody (Catalog # MP00107) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
References
Boster Bio
Anti-Phospho-Src (Y419) Monoclonal AntibodyMP00107
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Mouse, Rat
TargetSRC
  • SizePrice
Western blot analysis of Phospho-STAT3 (S727) expression in A431 cell lysate treated with EGF (P00007-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 monoclonal antibody (Catalog # P00007-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT3
Product group Antibodies
References
Boster Bio
Anti-Phospho-STAT3 (S727) Rabbit Monoclonal AntibodyP00007-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSTAT3
  • SizePrice
Western blot analysis of Phospho-Smad2 (S250) expression in (1) HeLa cell treated with alkaline phosphatase lysate; (2) HeLa cell lysate (P00090-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 monoclonal antibody (Catalog # P00090-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2
Product group Antibodies
References
Boster Bio
Anti-Phospho-Smad2 (S250) Rabbit Monoclonal AntibodyP00090-1
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Mouse, Rat
TargetSMAD2
  • SizePrice
Western blot analysis of Phospho-Stat5 (Y694) expression in A431 cell lysate treated with EGF (P01087-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT5A monoclonal antibody (Catalog # P01087-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT5A
Product group Antibodies
References
Boster Bio
Anti-Phospho-Stat5 (Y694) STAT5A Rabbit Monoclonal AntibodyP01087-1
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetSTAT5A
  • SizePrice
Western blot analysis of STAT3 expression in (1) A431 cell lysate; (2) Raji cell lysate.
Product group Antibodies
References
Boster Bio
Anti-STAT3 Rabbit Monoclonal AntibodyM00007
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSTAT3
  • SizePrice
Immunohistochemical analysis of paraffin-embedded mouse kidney, using Smad3 Antibody(M00059) SMAD3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD3 Antibody (M00059)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
References
Boster Bio
Anti-Smad3 Rabbit Monoclonal AntibodyM00059
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSMAD3
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human cervix cancer, using GLUT1 Antibody.
Product group Antibodies
References
Boster Bio
Anti-GLUT1 SLC2A1 Rabbit Monoclonal AntibodyM00163
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSLC2A1
  • SizePrice
Western blot analysis of SOX9 expression in SW480 cell lysate.
Product group Antibodies
References
Boster Bio
Anti-SOX9 Rabbit Monoclonal AntibodyM00177
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSOX9
  • SizePrice
Western blot analysis of SERPINA3 using anti-SERPINA3 antibody (M02312) in human plasma lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINA3 antigen affinity purified polyclonal antibody (Catalog # M02312) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
References
Boster Bio
Anti-alpha 1 Antichymotrypsin SERPINA3 Monoclonal AntibodyM02312
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetSERPINA3
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human pancreas, using Phospho-STAT3 (Y705) Antibody.
Product group Antibodies
References
Boster Bio
Anti-Phospho-STAT3 (Y705) Rabbit Monoclonal AntibodyP00007-2
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSTAT3
  • SizePrice
Immunofluorescent analysis of A549 cells treated with TGFβ, using Phospho-Smad3 (S423 + S425) Antibody.
Product group Antibodies
References
Boster Bio
Anti-Phospho-Smad3 (S423 + S425) Rabbit Monoclonal AntibodyP00059-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetSMAD3
  • SizePrice
Western blot analysis of Phospho-Smad2 (S255) expression in HeLa cell treated with Okadaic acid and Calyculin A lysate (P00090). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 monoclonal antibody (Catalog # P00090) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2
Product group Antibodies
References
Boster Bio
Anti-Phospho-Smad2 (S255) Rabbit Monoclonal AntibodyP00090
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSMAD2
  • SizePrice
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