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Search results: 19-c03

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Western blot analysis of Synuclein phosphorylation expression in Human fetal brain lysate (P00215-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNCA monoclonal antibody (Catalog # P00215-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNCA
Product group Antibodies
References
Boster Bio
Anti-Phospho-alpha Synuclein (S129) SNCA Rabbit Monoclonal AntibodyP00215-2
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetSNCA
  • SizePrice
Product group Antibodies
Biorbyt
SAH 4a antibody (HRP)ORB221067
ApplicationsELISA
  • SizePrice
Product group Antibodies
Biorbyt
Hrs (HGS) Mouse Monoclonal Antibody (19-5)ORB234014
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
  • SizePrice
Product group Antibodies
Biorbyt
P-Cadherin-3 CDH3-Antibody (Monoclonal, CH-19)ORB18255
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityChicken, Human, Mouse, Rabbit, Reptile, Rat
TargetCdh3
  • SizePrice
Product group Antibodies
Biorbyt
Cathepsin D Ctsd Monoclonal AntibodyORB18191
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Product group Antibodies
Biorbyt
H type 2 antibodyORB301955
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman
  • SizePrice
Product group Antibodies
OriGene
Cd5 Mouse Monoclonal Antibody [Clone ID: OX-19]SM258P
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityRat
TargetCd5
  • SizePrice
Product group Antibodies
OriGene
Cd5 Mouse Monoclonal Antibody [Clone ID: OX-19]SM258PS
ApplicationsFlow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry
ReactivityRat
TargetCd5
  • SizePrice
Immunohistochemistry staining of Paraffin Stomach tissue by MUC 5AC antibody (dilution: 1:100 - 1:500; visualization of staining: Cytoplasmic)
Product group Antibodies
OriGene
Mucin 5AC (MUC5AC) Mouse Monoclonal Antibody [Clone ID: MRQ-19]TA327703
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetMUC5AC
  • SizePrice
Product group Antibodies
OriGene
CCR5 Mouse Monoclonal Antibody [Clone ID: E11/19]TA386396
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman
TargetCCR5
  • SizePrice
Flow cytometry using the anti-CCR5 (phosphoserine 349) antibody E11/19 (TA386397). U937 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (TA385792; isotype control, black line) or the rabbit IgG1 version of E11/19 (TA386397, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Product group Antibodies
OriGene
CCR5 Rabbit Monoclonal Antibody [Clone ID: E11/19]TA386397
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman
TargetCCR5
  • SizePrice
Product group Antibodies
Antibodies.com
Anti-CD5 Antibody [OX-19] (FITC)A281406
ApplicationsFlow Cytometry
ReactivityRat
  • SizePrice
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