Bio-Connect

Search results: AMPK alpha 1

133 to 144 of 207.106 products
Sort by
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHuman
TargetPRKAA1
  • SizePrice
More sizes
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHamster, Human, Monkey, Mouse, Rat
TargetPRKAA1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityChicken, Human, Mouse, Rat
TargetPRKAA1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetPRKAA1
  • SizePrice
More sizes
Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityDrosophila
TargetPRKAA1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry
ReactivityBovine, Canine, Chicken, Equine, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Xenopus, Zebra Fish
TargetPRKAA1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
ReactivityHuman, Monkey, Mouse, Rat
TargetPRKAA1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetPRKAA1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetPRKAA1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPRKAA1
  • SizePrice
GTX130429 WB Image
Product group Antibodies
References
GeneTex
  • SizePrice
Western blot analysis of AMPK alpha 1 using anti-AMPK alpha 1 antibody (A00994-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PANC-1 whole cell lysates, Lane 2: human Jurkat whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPK alpha 1 antigen affinity purified polyclonal antibody (Catalog # A00994-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPK alpha 1 at approximately 63KD. The expected band size for AMPK alpha 1 is at 63KD.
Product group Antibodies
Boster Bio