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Search results: ASK1 MAP3K5 MEKK5

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Western blot analysis of ASK1 phosphorylation expression in TNF treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyTA325647
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP3K5
  • SizePrice
Figure 1. Immunoblot of anti-pS83 ASK1 antibodies shows specificity for phosphorylated human ASK1. Anti-pS83 (aa 76-87) antibody, generated by immunization with phospho peptide coupled to KLH, was tested by immunoblot against lysates of Cos-7 cells after transient transfection, separately, with 1) vector only, 2) recombinant HA-ASK1, and 3) recombinant human HA-ASK1 where S83 was substituted with an alanine residue. Cells were lysed 24 h post-transfection in 200 microL of 1x SDS-sample buffer, heated at 96°C for 5, and vortexed for 30 sec. Samples (10 microL each) were separated on a 12% SDS-PAGE gel and transferred to PVDF (Millipore) followed by blocking for 45 using TTBS supplemented with 5% non-fat dry milk. All incubations were performed at room temperature. In panel a) all samples were incubated with 10 ug/mL mouse anti-HA antibody for 45. After 5X washes with TTBS, reaction with ALP rabbit anti-mouse IgG at 200 ng/mL proceeded for 45 following again by washing as before. The blot was developed using BCIP/NBT. This blot demonstrates both recombinant transfections were successfully over-expressed in the Cos-7 cells. In panel b) all samples were incubated with a 1:1,000 dilution of ASK1 antibody for 45. The antibody was pre-incubated with non-phospho peptide prior to membrane incubation. After 5X washes with TTBS, reaction with HRP goat anti-rabbit IgG at 10 ng/mL proceeded for 45 following again by washing as before. The membrane was processed as before. Lane 2 shows strong specific staining of ASK1. Lane 3, where S83 was replaced with alanine, shows greatly diminished staining. In panel c) all samples were incubated with a 1:1,000 dilution of ASK1 antibody as before except the antibody was preincubated with phospho peptide prior to membrane incubation. No staining is observed after phospho peptide blocking occurs.
Product group Antibodies
OriGene
ASK1 (MAP3K5) pSer83 Rabbit Polyclonal AntibodyR1172
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetMAP3K5
  • SizePrice
Western blot (WB) analysis of p-ASK1 (pSer83) antibody in extracts from MDA-MB-435 cells treated with TNF-a.
Product group Antibodies
OriGene
ASK1 (MAP3K5) pSer83 Rabbit Polyclonal AntibodyAP01526PU-N
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetMAP3K5
  • SizePrice
Western blot analysis of ASK1 antibody (pSer966) Cat.-No AP01527PU-N
Product group Antibodies
OriGene
ASK1 (MAP3K5) pSer966 Rabbit Polyclonal AntibodyAP01527PU-N
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetMAP3K5
  • SizePrice
Figure 2. Western blot analysis of extracts from K562 cells using ASK1 (phospho-Ser83) antibody.
Product group Antibodies
OriGene
ASK1 (MAP3K5) pSer83 Rabbit Polyclonal AntibodyAP02455PU-N
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetMAP3K5
  • SizePrice
Figure 2. Western blot analysis of extracts from K562 cells using ASK1 (phospho-Ser83) antibody.
Product group Antibodies
OriGene
ASK1 (MAP3K5) pSer83 Rabbit Polyclonal AntibodyAP02455PU-S
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetMAP3K5
  • SizePrice
Figure 2. Western blot analysis of extracts from various cells using ASK1 antibody (Phospho-Ser966).
Product group Antibodies
OriGene
ASK1 (MAP3K5) pSer966 Rabbit Polyclonal AntibodyAP02456PU-N
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetMAP3K5
  • SizePrice
Figure 2. Western blot analysis of extracts from various cells using ASK1 antibody (Phospho-Ser966).
Product group Antibodies
OriGene
ASK1 (MAP3K5) pSer966 Rabbit Polyclonal AntibodyAP02456PU-S
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetMAP3K5
  • SizePrice
Immunohistochemistry (IHC) analyzes of p-ASK1 antibody (Cat.-No.: AP20924PU-N) in paraffin-embedded human lung adenocarcinoma tissue.
Product group Antibodies
OriGene
ASK1 (MAP3K5) pSer83 Rabbit Polyclonal AntibodyAP20924PU-N
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetMAP3K5
  • SizePrice
Western blot (WB) analysis of p-ASK1 antibody (Cat.-No.: AP20925PU-N) in paraffin-embedded human breast carcinoma tissue.
Product group Antibodies
OriGene
ASK1 (MAP3K5) pSer966 Rabbit Polyclonal AntibodyAP20925PU-N
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP3K5
  • SizePrice
Product group Antibodies
MyBioSource
ASK1 (MEKK5/MAP3K5) (S1033) Rabbit pAbMBS8558360
  • SizePrice
Western blot analysis using MAP3K5 mAb against human MAP3K5 (AA: 922-1108) recombinant protein. (Expected MW is 46.5 kDa)
Product group Antibodies
OriGene
ASK1 (MAP3K5) Mouse Monoclonal Antibody [Clone ID: 2E4]AM06637SU-N
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetMAP3K5
  • SizePrice
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