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Search results: ASK1 MAP3K5 MEKK5

157 to 168 of 1.110 products
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
MAP3K5 / ASK1 Antibody Blocking PeptideLS-E9363
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
MAP3K5 / ASK1 Antibody Blocking PeptideLS-E9364
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Product group Antibodies
LifeSpan BioSciences
MAP3K5 / ASK1 Antibody (phospho-Ser966)LS-C825226
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
MAP3K5 / ASK1 Antibody (phospho-Ser83)LS-C826120
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Product group Antibodies
Boster Bio
Anti-ASK1 (phospho-S83) MAP3K5 AntibodyA00929S83
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetMAP3K5
  • SizePrice
Product group Antibodies
Boster Bio
Anti-ASK1 (phospho-S966) MAP3K5 AntibodyA00929S966
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetMAP3K5
  • SizePrice
Product group Antibodies
Boster Bio
Anti-ASK1 (Ab-966) MAP3K5 AntibodyA00929-2
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP3K5
  • SizePrice
Product group Antibodies
Boster Bio
Anti-ASK1 (phospho-S966) MAP3K5 AntibodyA00929S966-1
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP3K5
  • SizePrice
Product group Antibodies
Boster Bio
Anti-ASK1 MAP3K5 Rabbit Monoclonal AntibodyM00929-30UL
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetMAP3K5
  • SizePrice
Figure 1. Immunoblot of anti-pS83 ASK1 antibodies shows specificity for phosphorylated human ASK1. Anti-pS83 (aa 76-87) antibody, generated by immunization with phospho peptide coupled to KLH, was tested by immunoblot against lysates of Cos-7 cells after transient transfection, separately, with 1) vector only, 2) recombinant HA-ASK1, and 3) recombinant human HA-ASK1 where S83 was substituted with an alanine residue. Cells were lysed 24 h post-transfection in 200 microL of 1x SDS-sample buffer, heated at 96°C for 5, and vortexed for 30 sec. Samples (10 microL each) were separated on a 12% SDS-PAGE gel and transferred to PVDF (Millipore) followed by blocking for 45 using TTBS supplemented with 5% non-fat dry milk. All incubations were performed at room temperature. In panel a) all samples were incubated with 10 ug/mL mouse anti-HA antibody for 45. After 5X washes with TTBS, reaction with ALP rabbit anti-mouse IgG at 200 ng/mL proceeded for 45 following again by washing as before. The blot was developed using BCIP/NBT. This blot demonstrates both recombinant transfections were successfully over-expressed in the Cos-7 cells. In panel b) all samples were incubated with a 1:1,000 dilution of ASK1 antibody for 45. The antibody was pre-incubated with non-phospho peptide prior to membrane incubation. After 5X washes with TTBS, reaction with HRP goat anti-rabbit IgG at 10 ng/mL proceeded for 45 following again by washing as before. The membrane was processed as before. Lane 2 shows strong specific staining of ASK1. Lane 3, where S83 was replaced with alanine, shows greatly diminished staining. In panel c) all samples were incubated with a 1:1,000 dilution of ASK1 antibody as before except the antibody was preincubated with phospho peptide prior to membrane incubation. No staining is observed after phospho peptide blocking occurs.
Product group Antibodies
OriGene
ASK1 (MAP3K5) pSer83 Rabbit Polyclonal AntibodyR1172
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetMAP3K5
  • SizePrice
Figure 2. Western blot analysis of extracts from K562 cells using ASK1 (phospho-Ser83) antibody.
Product group Antibodies
OriGene
ASK1 (MAP3K5) pSer83 Rabbit Polyclonal AntibodyAP02455PU-N
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetMAP3K5
  • SizePrice
Figure 2. Western blot analysis of extracts from K562 cells using ASK1 (phospho-Ser83) antibody.
Product group Antibodies
OriGene
ASK1 (MAP3K5) pSer83 Rabbit Polyclonal AntibodyAP02455PU-S
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetMAP3K5
  • SizePrice
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