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Search results: ASK1 MAP3K5 MEKK5

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Western blot analysis of extracts of various cell lines, using Phospho-MAP3K5-S83 antibody (TA378272) at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour, treated by TNF-alpha (20ng/mL) for 30 minutes after serum-starvation overnight or treated by ATP (5mM) at 30°C for 1 hour. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 60s.
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyTA378272
ApplicationsWestern Blot
ReactivityHuman
TargetMAP3K5
  • SizePrice
Western blot analysis of extracts of HeLa cells, using Phospho-MAP3K5-T918 antibody (TA378273) at 1:1000 dilution. HeLa cells were treated by UV for 15-30 minutes. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA.
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyTA378273
ApplicationsWestern Blot
ReactivityHuman
TargetMAP3K5
  • SizePrice
Western blot analysis of extracts of Jurkat cells, using Phospho-MAP3K5-S967 antibody (TA378275) at 1:1000 dilution.Jurkat cells were treated by CIP(20uL/400ul) at 37°C for 1 hour.Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37°C for 2 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Enhanced Kit . - Exposure time: 180s.
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyTA378275
ApplicationsWestern Blot
ReactivityHuman
TargetMAP3K5
  • SizePrice
Western blot analysis of MAP3K5 (arrow) using rabbit polyclonal MAP3K5 Antibody (Cat.#TA302148).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP3K5 gene (Lane 2) (Origene Technologies).
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyTA302148
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetMAP3K5
  • SizePrice
Western blot analysis of ASK1 in HeLa lysates using ASK1 antibody.
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyTA384716
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetMAP3K5
  • SizePrice
Western blot analysis of ASK1 expression in MDA-MB-435 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyTA325644
ApplicationsWestern Blot
ReactivityHuman
TargetMAP3K5
  • SizePrice
Western blot analysis of ASK1 expression in RAW264.7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyTA325645
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP3K5
  • SizePrice
Western blot analysis of ASK1 in SW1353 whole cell lysate with ASK1 antibody at 1:500 dilution.
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyTA305959
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman
TargetMAP3K5
  • SizePrice
Western blot analysis of ASK1 in SW1353 cell lysate with ASK1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyTA306284
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse
TargetMAP3K5
  • SizePrice
Western blot analysis of ASK1 in 3T3 cell lysate with ASK1 antibody at (A) 1 and (B) 2 ug/ml.
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyTA306285
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetMAP3K5
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using ASK1 (Phospho-Ser966) Antibody.
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyTA384715
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP3K5
  • SizePrice
Western blot analysis of ASK1 phosphorylation expression in TNF-a treated MDA-MB-435 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
ASK1 (MAP3K5) Rabbit Polyclonal AntibodyTA325646
ApplicationsWestern Blot
ReactivityHuman
TargetMAP3K5
  • SizePrice
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