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Search results: ATG13

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Product group Antibodies
MyBioSource
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Product group Antibodies
ABclonal
TargetATG13
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Product group Antibodies
Abbexa
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Western blot using Rocklands affinity purified anti-ATG13 antibody shows detection of ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13), right lane, but not in the left lane in which was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. Detection is demonstrated at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blotto (p/n B501-0500) and probed with the primary antibody at 1microg/mL overnight at 4°C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer (p/n MB-070) for 45 minutes at room temperature. In collaboration with Charles Dorsey at Eli Lilly, Indianapolis, IN and John Cleveland at Scripps, Jupiter, FL.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetATG13
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Western blot analysis of ATG13 in rat heart tissue lysate with ATG13 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetATG13
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Western blot using Rocklands affinity purified anti-ATG13 pS318 antibody shows detection of phosphorylated ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13). In the left lane was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. The right lane contains the 293T Ulk1 + ATG13 lysate and shows detection at approximately 57 kDa. The antibody was purified and resolved by SDS-PAGE, then transferred to nitrocellulose membrane. The membrane was blocked with 5% Blotto (p/n B501-0500) and probed with the primary antibody at 1microg/mL overnight at 4°C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000 in detection buffer (p/n MB-070) for 45 minutes at room temperature. In collaboration with Charles Dorsey at Eli Lilly, Indianapolis, IN and John Cleveland at Scripps, Jupiter, FL.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetATG13
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Western blot analysis of extracts of various cell lines, using ATG13 antibody.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetATG13
  • SizePrice
WB using Anti-ATG13 antibody shows detection of ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13), right lane, but not in the left lane in which was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. Detection is demonstrated at approximately 57 kDa. primary antibody was used at 1ug/mL overnight at 4°C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetATG13
  • SizePrice
WB Suggested Anti-ATG13 Antibody; Titration: 1.0 ug/ml; Positive Control: 293T Whole Cell There is BioGPS gene expression data showing that ATG13 is expressed in HEK293T
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetATG13
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WB Suggested Anti-ATG13 Antibody Titration: 1.0 ug/ml Positive Control: PANC1 Whole CellATG13 is strongly supported by BioGPS gene expression data to be expressed in Human PANC1 cells
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetATG13
  • SizePrice
WB using Anti-ATG13 pS318 antibody shows detection of phosphorylated ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13). In the left lane was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. The right lane contains the 293T Ulk1 + ATG13 lysate and shows detection at approximately 57 kDa.The primary antibody was used at 1ug/mL. Goat Anti-Rabbit HRP was used at 1:5000.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetATG13
  • SizePrice
Product group Antibodies
MyBioSource
  • SizePrice