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Figure 1. Western blot analysis of Apg3 using anti-Apg3 antibody (M01768). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apg3 antigen affinity purified monoclonal antibody (Catalog # M01768) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apg3 at approximately 40 kDa. The expected band size for Apg3 is at 36 kDa.
Product group Antibodies
References
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetATG3
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Product group Molecular Biology
Sino Biological
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Product group Molecular Biology
Sino Biological
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Product group Molecular Biology
Sino Biological
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Product group Molecular Biology
Sino Biological
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Product group Chemicals
MedChem Express
CAS Number333351-38-5
Estimated Purity99.31
Molecular Weight327.40
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Product group Chemicals
TargetMol Chemicals
Molecular Weight327.4
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Product group Antibodies
Abbexa
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
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Product group Antibodies
Abbexa
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
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Product group Antibodies
Abbexa
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
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Product group Antibodies
Abbexa
ApplicationsELISA, ImmunoHistoChemistry
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Product group Antibodies
Abbexa
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
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