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Search results: BIM

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Human Bim ELISA Kit
Product group Assays
Antibodies.com
ReactivityHuman
  • SizePrice
Human Bim ELISA Kit
Product group Assays
Antibodies.com
Assay Sample TypeTissue homogenates, cell lysates and other biological fluids.
ReactivityHuman
  • SizePrice
Product group Antibodies
Assay Genie
ReactivityHuman
TargetNR1I3
  • SizePrice
Product group Antibodies
Abbexa
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
ReactivityMouse
TargetBcl2l11
  • SizePrice
Product group Antibodies
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
ReactivityMouse
TargetBcl2l11
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetBcl2l11
  • SizePrice
Figure 1. Western blot analysis of Bim/Bcl2l11 using anti-Bim/Bcl2l11 antibody (A01552-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bim/Bcl2l11 antigen affinity purified polyclonal antibody (Catalog # A01552-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bim/Bcl2l11 at approximately 22 kDa. The expected band size for Bim/Bcl2l11 is at 22 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityMouse
TargetBcl2l11
  • SizePrice
Figure 1. Western blot analysis of Bim/Bcl2l11 using anti-Bim/Bcl2l11 antibody (A01552-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bim/Bcl2l11 antigen affinity purified polyclonal antibody (Catalog # A01552-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bim/Bcl2l11 at approximately 22 kDa. The expected band size for Bim/Bcl2l11 is at 22 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityMouse
TargetBcl2l11
  • SizePrice
Figure 1. Western blot analysis of Bim/Bcl2l11 using anti-Bim/Bcl2l11 antibody (A01552-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bim/Bcl2l11 antigen affinity purified polyclonal antibody (Catalog # A01552-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bim/Bcl2l11 at approximately 22 kDa. The expected band size for Bim/Bcl2l11 is at 22 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityMouse
TargetBcl2l11
  • SizePrice
Figure 1. Western blot analysis of Bim/Bcl2l11 using anti-Bim/Bcl2l11 antibody (A01552-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bim/Bcl2l11 antigen affinity purified polyclonal antibody (Catalog # A01552-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bim/Bcl2l11 at approximately 22 kDa. The expected band size for Bim/Bcl2l11 is at 22 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityMouse
TargetBcl2l11
  • SizePrice
Figure 1. Western blot analysis of Bim/Bcl2l11 using anti-Bim/Bcl2l11 antibody (A01552-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bim/Bcl2l11 antigen affinity purified polyclonal antibody (Catalog # A01552-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bim/Bcl2l11 at approximately 22 kDa. The expected band size for Bim/Bcl2l11 is at 22 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityMouse
TargetBcl2l11
  • SizePrice